Nile Red Fluorescence Wavelength

Nile Red: A Versatile Fluorescent Tool for Biological Applications

Introduction

Nile Red is a fluorescent compound that has gained prominence in biological research due to its unique properties and versatility. With an excitation peak at 559 nm and an emission peak at 635 nm, Nile Red exhibits fluorescence enhancement and large Stokes shifts in nonpolar solvents compared to polar solvents.

Fluorescence Properties

In water and other polar solvents, Nile Red is almost nonfluorescent. However, upon interaction with nonpolar environments, such as lipid droplets, its fluorescence dramatically increases. This property makes Nile Red an ideal probe for visualizing and quantifying neutral lipids in cells and tissues.

Applications in Microalgae Research

WEB Nile Red has proven particularly useful in monitoring neutral lipid content in microalgae. The excitation wavelength of 562 nm and observation wavelengths of 630 nm and 700 nm allow for specific detection of neutral lipids. This technique has enabled researchers to rapidly assess lipid accumulation and production in microalgae cultures.

Efficiency and Specificity

Nile Red (NR) is renowned for its efficiency as a fluorescent dye. Its well-pronounced solvatochromism, meaning it exhibits different fluorescence properties depending on the solvent, makes it a versatile probe for a wide range of biological applications.

Conclusion

Nile Red's unique fluorescence properties and versatility have made it an indispensable tool in biological research. From visualizing lipid droplets to monitoring lipid content in microalgae, Nile Red has provided valuable insights and contributed to our understanding of cellular processes and biological systems.